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HPV DNA Test Alone and HPV DNA Test Combined with VIA as Screening Test for Cervical Cancer
Shabnom Mostofa Purobi1, Ashrafunnesa1, Shirin Akter Begum1
1Department of Obstetrics and Gynecology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh.
Journal of Pharmacy & Bioallied Sciences
|March 19, 2026
Summary
Combining HPV DNA testing with visual inspection with acetic acid (VIA) significantly enhances cervical cancer screening accuracy in Bangladesh. This dual approach offers improved specificity and predictive value, crucial for effective early detection in low-resource settings.
Area of Science:
- Oncology
- Virology
- Public Health
Background:
- Cervical cancer (CC) is a major global health concern for women, primarily caused by persistent high-risk human papillomavirus (HPV) infections.
- Effective screening strategies are vital for reducing CC mortality, particularly in resource-limited regions.
Purpose of the Study:
- To evaluate and compare the diagnostic accuracy of HPV DNA testing alone versus its combination with visual inspection with acetic acid (VIA) for cervical cancer screening.
- To assess the effectiveness of these methods in the context of Bangladesh.
Main Methods:
- A cross-sectional analytical study involving 400 women was conducted.
- Participants underwent HPV DNA testing, VIA, and colposcopy for diagnostic comparison.
- Data analysis was performed using SPSS version 26.0.
Main Results:
- The combined HPV DNA and VIA approach demonstrated 80% sensitivity, 100% specificity, and 100% positive predictive value (PPV), with 99.5% overall accuracy.
- Single HPV DNA testing showed 80% sensitivity and 88.8% PPV, while VIA had 100% sensitivity but a low PPV (22.7%).
- Colposcopy confirmed 10 cases (2.5%) of cervical cancer.
Conclusions:
- Combining HPV DNA testing with VIA significantly enhances diagnostic specificity and predictive value for cervical cancer screening.
- This combined strategy represents a highly effective approach for CC screening, especially in low-resource settings like Bangladesh.
