Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Central obesity may affect bone development in adolescents: association between abdominal obesity index ABSI and adolescent bone mineral density.

BMC endocrine disorders·2024

Same author

The Impact of Renewable Energy Development on Regional Carbon Emission Reduction: Based on the Spatio-Temporal Analysis of 30 Provinces in China.

Environmental management·2024

Same author

Investigation on the Effectiveness of Digital Scanning Combined with Reverse Engineering Technology in Demonstrating Full-Crown Tooth Preparation.

The International journal of prosthodontics·2024

Same author

Legionella maintains host cell ubiquitin homeostasis by effectors with unique catalytic mechanisms.

Research square·2024

Same author

A fast relay feedback auto-tuning tilt-integral-derivative (TID) controller method with the fractional-order Ziegler-Nichols approach.

ISA transactions·2024

Same author

Experimental validation of the accuracy of robotic-assisted radioactive seed implantation for tumor treatment.

Journal of robotic surgery·2024

Same journal

Sinomenine Regulates the TRIM32/IRF1/TRAF6 Axis to Inhibit Pyroptosis in Atopic Dermatitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same journal

Urolithin A Mitigates Renal Fibrosis by Promoting Fatty Acid Oxidation Through Orchestrating β-Catenin Signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same journal

MiR-124 Inhibits Lipid Deposition in Mouse Liver by Targeting the Trib3/Hnf4α Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same journal

TCN2 Drives Psoriasis-Like Inflammation and Keratinocyte Hyperproliferation, Correlating With IL-1β and STAT3 Activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same journal

The Synthetic Melanocortin Agonist NDP-MSH Ameliorates THSD7A-Associated Membranous Nephropathy in an Active Immunization Mouse Model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same journal

Brain-Derived Neurotrophic Factor and Associated Signaling in Kidney Diseases.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Identification and Validation of miR-206-3p Targeting WT-1 Promotes Membranous Nephropathy Through a Comprehensive

Xiaowan Wang^1,2,3,4, Fangqiang Zhou^1,2, Chunmei Fu^1,2

¹State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology

|March 19, 2026

Summary

This summary is machine-generated.

MicroRNAs (miRs) are implicated in membranous nephropathy (MN). This study identifies miR-206 as a key player targeting WT-1, offering potential therapeutic strategies for MN by inhibiting podocyte apoptosis.

Keywords:

bioinformatics and machine learning membranous nephropathy miR‐206‐3p podocyte apoptosis the C‐MIP/Nephrin/PI3K pathway

More Related Videos

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Related Experiment Videos

Last Updated: Mar 21, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

Nephrology
Molecular Biology
Genetics

Background:

Membranous nephropathy (MN) is a leading cause of kidney failure with limited treatment options.
The role of microRNAs (miRs) in MN pathogenesis is largely unknown.
Identifying novel molecular targets is crucial for developing effective MN therapies.

Purpose of the Study:

To identify key microRNAs (miRs) and molecular mechanisms involved in membranous nephropathy (MN).
To investigate the role of specific miRs and their target genes in podocyte apoptosis.
To explore potential therapeutic targets for MN.

Main Methods:

Utilized public databases (GEO, miRbase, TargetScan) and bioinformatics tools (WGCNA, LASSO).
Employed machine learning and disease-related analyses to identify biomarkers.
Validated findings in a rat model of nephropathy and in vitro podocyte injury models.

Main Results:

Identified 13 upregulated differentially expressed miRs (DEmiRs) and 58 positively correlated miRs in MN.
Pinpointed miR-206 and WT-1 as key biomarkers for MN.
Confirmed upregulation of miR-206-3p and downregulation of WT-1 in apoptotic podocytes.
Elucidated the C-MIP/Nephrin/PI3K pathway as the mechanism for miR-206-3p targeting WT-1.
Demonstrated that inhibiting miR-206-3p restores WT-1 expression and reduces podocyte apoptosis.

Conclusions:

miR-206-3p plays a critical role in mediating podocyte apoptosis in membranous nephropathy (MN) by targeting WT-1.
The C-MIP/Nephrin/PI3K pathway is involved in the miR-206-3p/WT-1 interaction.
Inhibition of miR-206-3p represents a potential therapeutic strategy for MN.