Protocol for measuring lipid membrane fluidity in human iPSC-derived neural cells
Related Experiment Video
Updated: Mar 21, 2026

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies
Published on: July 28, 2023
Satoshi Morita1, Takayuki Kondo2, Haruhisa Inoue2
1iPSC-based Drug Discovery and Development Team, RIKEN BioResource Research Center (BRC), Kyoto 619-0237, Japan; Institute for Science of Life, Suntory Wellness Ltd., Kyoto 619-0284, Japan; Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
Abstract:
Here, we present a protocol for describing the differentiation of human cortical neurons from induced pluripotent stem cells (iPSCs) and the subsequent analysis of lipid membrane fluidity using the LipiORDER fluorescent probe. We describe steps for embryoid body formation, neuronal induction with adherent culture maturation, and live-cell membrane fluidity measurement. This approach allows for investigation of the effects of lipid membrane fluidity on neuronal function and pathophysiology. For complete details of the use and execution of this protocol, please refer to Morita et al.1.

