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Association analysis and in silico functional predictions of RMDN2 variants in chickens
Ashi Li1,2, Yuechen Liao1,2,3, Yangqiwen Luo1,2
1Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Animal Bioscience
|March 19, 2026
Summary
This study identified novel single-nucleotide polymorphisms (SNPs) in the RMDN2 gene associated with chicken laying traits, offering potential for marker-assisted breeding. Further research is needed to validate these findings for practical application.
Area of Science:
- Animal Genetics
- Molecular Biology
- Poultry Science
Background:
- Microtubule dynamics regulator protein 2 (RMDN2) is crucial for cell division and cytoskeleton maintenance.
- Previous studies identified RMDN2 as a candidate gene influencing chicken follicle development.
- Understanding RMDN2's role can enhance chicken laying traits and breeding strategies.
Purpose of the Study:
- To explore single-nucleotide polymorphisms (SNPs) in the RMDN2 gene.
- To perform phylogenetic analysis of RMDN2.
- To assess RMDN2 sequence characteristics for insights into chicken laying traits.
Main Methods:
- Identified SNPs in the RMDN2 coding sequence (CDS) of an F2 resource population.
- Utilized bioinformatics tools to predict the impact of SNP mutations on RMDN2 protein structure and function.
- Constructed a phylogenetic tree to analyze RMDN2 evolution and its relation to laying traits.
Main Results:
- Discovered four novel exonic SNPs (SNP1-SNP4) in RMDN2.
- Heterozygous genotypes of SNP1, SNP3, and SNP4 associated with increased egg number (EN66).
- Heterozygous genotype of SNP2 associated with higher body weight at first egg (BWFE).
- Haplotype H1H1 linked to lower BWFE, body weight at 105 days (BW105), and first egg weight (FEW).
- Missense mutations in SNP1, SNP2, and SNP3 may affect protein stability and function.
- Phylogenetic analysis showed high conservation within orders but divergence across different taxonomic orders.
Conclusions:
- The identified RMDN2 SNPs enrich the candidate gene pool for chicken laying traits.
- These findings provide a theoretical basis for molecular marker-assisted breeding in poultry.
- In vivo and in vitro validation are necessary to strengthen the application of these findings.
