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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Diagnostic and genomic characterization of an imported chikungunya virus case in Ningxia Hui Autonomous Region,
Jianxin Pei1, Jingting Wang1, Ling Niu2
1Ningxia Hui Autonomous Region Academy of Preventive Medicine, Ningxia Center for Disease Control and Prevention, Yinchuan 750004, China.
Abstract:
To analyze the first imported case of chikungunya virus (CHIKV) in Ningxia Hui Autonomous Region (Ningxia), China, and to provide insights for the prevention and control of emerging infectious diseases. An epidemiological investigation and viral detection was performed via quantitative reverse transcription polymerase chain reaction (qRT-PCR). The virus was further characterized using whole-genome sequencing and phylogenetic analysis. A 26-year-old female was confirmed to be infected with CHIKV. Genotypic analysis identified the East/Central/South African (ECSA) Indian Ocean Lineage (IOL), specifically the ECSA2 sub-lineage. A key mutation (E1-A226V), known to enhance viral adaptability to Aedes albopictus, was identified. Phylogenetic analysis revealed 99.21% nucleotide similarity to a CHIKV strain isolated in Foshan City, Guangdong Province, in 2025. No Ae. aegypti or Ae. albopictus were found in Ningxia. This imported case of CHIKV in Ningxia, genetically linked to the ECSA-IOL lineage carrying the E1-A226V mutation, underscores the risk of introduction and potential local transmission of arboviruses via domestic travel. Enhanced surveillance, timely diagnosis, and effective management of imported cases are essential for preventing outbreaks.
