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Related Experiment Video

Updated: Mar 22, 2026

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Recent advances in protein modification-enabled potential therapeutics.

Lizhen Wei¹, Kainan Liang¹, Yang Zhou¹

¹Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, PR China.

Current Opinion in Chemical Biology

|March 20, 2026

Summary

This summary is machine-generated.

Recent advances in bioconjugation techniques enable precise engineering of protein therapeutics, improving stability and efficacy. Future directions focus on scalable platforms and AI-driven design for enhanced drug delivery and reduced immunogenicity.

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Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Published on: November 7, 2019

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Last Updated: Mar 22, 2026

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

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Published on: February 27, 2020

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Published on: November 7, 2019

Area of Science:

Biotechnology and Pharmaceutical Sciences
Protein Engineering
Drug Development

Background:

Protein therapeutics have advanced from native biologics to engineered agents, addressing limitations like instability and immunogenicity.
Early strategies (PEGylation, albumin fusion, ADCs) showed clinical value but faced challenges in specificity, heterogeneity, and payload diversity.
Recent bioconjugation innovations offer solutions for site-specific modifications and enhanced therapeutic properties.

Purpose of the Study:

To review recent advancements in modified protein therapeutics over the past two years.
To highlight key developments in antibody-drug conjugates (ADCs), cytokines, and proximity-enabled covalent therapeutics.
To outline future directions in protein therapeutic engineering.

Main Methods:

Review of recent literature on bioconjugation techniques and modified protein therapeutics.
Categorization of advancements in ADCs, cytokines, and proximity-enabled covalent therapeutics.
Analysis of emerging trends and future research directions.

Main Results:

Bioconjugation techniques (chemoselective reactions, enzymatic labeling, glycan engineering, genetic code expansion) enable site-specific and homogeneous modifications.
These advancements improve batch consistency, controlled payload release, and multi-functionality of protein therapeutics.
Key developments in ADCs, cytokines, and proximity-enabled covalent therapeutics were highlighted.

Conclusions:

Site-specific bioconjugation is crucial for overcoming limitations in current protein therapeutics.
Future research should focus on scalable platforms, immunogenicity management, and delivery optimization.
Artificial intelligence is poised to accelerate protein design and reaction manipulation for next-generation therapeutics.