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Updated: Mar 23, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Compressive multi-beam scanning transmission electron microscopy
Akira Yasuhara1, Takumi Sannomiya2, Ryoichi Horisaki3
1JEOL Ltd., 3-1-2 Musashino, Akishima, Tokyo 196-8558, Japan.
Abstract:
We demonstrate a multi-beam scanning transmission electron microscopy (STEM) imaging that integrates down-sampling with super-resolution image reconstruction via a compressive sensing framework. A custom condenser aperture with six randomly positioned circular holes is employed to produce a multi-beam STEM probe, with the beam shape and distribution tuned through defocus. While the raw multi-beam images exhibit overlapping patterns, reconstruction using Adam optimization and total variation normalization yields high-fidelity images that closely reproduce the original sample structures, even from substantially down-sampled data. The proposed approach offers a pathway toward significant acceleration of such techniques through multibeam sparse sampling and computational reconstruction potentially useful for the analytical scanning methods in general.
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