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Complete Genome Annotation of Mycobacteriophage Kremtemulon
Cole R Jirsa1, Bethany M Wise1, Kaylia Edwards1
1Department of Science Education, Howard Hughes Medical Institute, Chevy Chase, Maryland, United States.
Micropublication Biology
|March 23, 2026
Summary
Kremtemulon, a siphovirus infecting Mycobacterium smegmatis, has a 51,438 bp genome. Its genetic makeup suggests it is a temperate phage, capable of integrating into the host genome.
Area of Science:
- Bacteriology
- Virology
- Genomics
Background:
- Mycobacterium smegmatis is a common model organism for studying mycobacteria.
- Bacteriophages, viruses that infect bacteria, play crucial roles in microbial ecosystems.
- Siphoviruses are a common morphological type of bacteriophage.
Purpose of the Study:
- To characterize the bacteriophage Kremtemulon isolated from Mycobacterium smegmatis.
- To determine the genomic features and potential lifestyle of Kremtemulon.
Main Methods:
- Isolation and characterization of bacteriophage Kremtemulon from Mycobacterium smegmatis.
- Genome sequencing and analysis to identify genes and functional elements.
- Plaque morphology and genetic analysis to infer phage lifestyle.
Main Results:
- Kremtemulon is a siphovirus with a genome size of 51,438 bp.
- It encodes 87 putative genes, with 36 having predicted functions.
- Gene content places Kremtemulon in Cluster A, Subcluster A4.
- The phage forms turbid plaques and possesses an immunity repressor and tyrosine integrase.
Conclusions:
- Kremtemulon is a temperate siphovirus infecting Mycobacterium smegmatis.
- Its genetic elements support a lysogenic lifecycle, including integration into the host genome.
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