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Updated: Jul 13, 2026

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
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Genome sequence of protease-free Bacillus subtilis KO7-S strain created for protein production
Ankur Bothra1, Ryan M Routsong2, Daniel R Zeigler3
1Microbial Pathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Abstract:
Bacillus strains efficiently secrete heterologous recombinant proteins as well as multiple proteases, with the latter destroying the former. Strains deleted of multiple secreted proteases are, therefore, preferred as protein expression hosts. We report the genome sequence of one such easily available strain, Bacillus subtilis KO7-S.
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