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Updated: Mar 6, 2026

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Genome sequence of protease-free Bacillus anthracis BH500 strain created for protein production
Ankur Bothra1, Ryan M Routsong2, Andrei P Pomerantsev1
1Microbial Pathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Abstract:
Bacillus strains efficiently secrete heterologous recombinant proteins as well as multiple proteases, with the latter destroying the former. Strains deleted of multiple secreted proteases are therefore preferred as protein expression hosts. We report the genome sequence of one such easily available strain, Bacillus anthracis BH500.

