Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

33.8K
Overview
33.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combined effects of benzotriazole ultraviolet stabilizers and cadmium on physiological performance of marine dinoflagellate Akashiwo sanguinea.

Aquatic toxicology (Amsterdam, Netherlands)·2025
Same author

Omics analysis reveals key genes mediating herbicide resistance in Digitaria sanguinalis.

Pesticide biochemistry and physiology·2025
Same author

Tissue Distribution and Abundance of the Parasitic Dinoflagellate <i>Hematodinium perezi</i> in Naturally Infected <i>Portunus trituberculatus</i>.

Pathogens (Basel, Switzerland)·2025
Same author

The microspores of the parasitic dinoflagellate Hematodinium perezi infect crustacean hosts via waterborne transmission.

Journal of invertebrate pathology·2025
Same author

Isolation and characterization of Polaribacter sargassicola sp. nov. and Algibacter ulvanivorans sp. nov.: two novel species with ability to degrade algal polysaccharides.

Antonie van Leeuwenhoek·2025
Same author

Establishment of CARD-FISH and Dot-ELISA Assays for Diagnosis of the Parasitic Dinoflagellate Hematodinium perezi.

Journal of fish diseases·2025

Related Experiment Video

Updated: Mar 25, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

1.8K

Design and Evaluation of Universal Multi-Epitope Vaccines Against Vibrio Infections Using a Single/Multi-Target

Jingsheng Lun1,2,3, Peng Zheng1,2,3, Caiwen Li1

  • 1Department of Biology, College of Science, Shantou University, Shantou, China.

Journal of Fish Diseases
|March 24, 2026
PubMed
Summary

Novel multi-derived epitope vaccines offer broad protection against diverse Vibrio species. The multi-target vaccine (EVK-20) demonstrated superior efficacy compared to single-target (EVK-10) and single-derived controls.

Keywords:
cross‐immunoprotectionmulti‐epitope vaccineouter membrane proteinsuniversal vaccinevibrio species

More Related Videos

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
10:58

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

8.1K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.1K

Related Experiment Videos

Last Updated: Mar 25, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

1.8K
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
10:58

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

8.1K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.1K

Area of Science:

  • Veterinary immunology
  • Aquaculture health
  • Bacterial pathogenesis

Background:

  • Vibrio infections pose a significant threat to aquaculture sustainability.
  • Conventional Vibrio vaccines struggle with genetic diversity and antigenic variation.
  • Developing broad-spectrum vaccines against Vibrio is a major challenge.

Purpose of the Study:

  • To develop a novel vaccine strategy for broad-spectrum protection against Vibrio species.
  • To evaluate the efficacy of single/multi-target multi-derived epitope vaccines.
  • To compare the protective immunity conferred by different vaccine designs.

Main Methods:

  • Designed and constructed two multi-epitope vaccines: EVK-10 (single-target multi-derived) and EVK-20 (multi-target multi-derived) using conserved epitopes from Vibrio outer membrane proteins (OMPs).
  • Immunized zebrafish with the constructed vaccines and assessed antibody responses.
  • Conducted challenge trials using five heterologous Vibrio strains to evaluate vaccine efficacy and relative percent survival (RPS).

Main Results:

  • Both EVK-10 and EVK-20 induced substantial antibody responses in zebrafish.
  • EVK-10 and EVK-20 provided broad cross-immunoprotection against five heterologous Vibrio strains.
  • EVK-20 (multi-target) showed superior RPS (up to 75.0%) compared to EVK-10 (up to 67.9%) and the single-derived control (up to 85.5% against homologous/closely related strains but lower against distant ones).

Conclusions:

  • The multi-derived epitope strategy confers broader cross-protection against diverse Vibrio species than single-derived vaccines.
  • The multi-target approach (EVK-20) provides stronger and more redundant immunity than the single-target design (EVK-10).
  • The single/multi-target multi-derived epitope strategy is a promising platform for developing universal Vibrio vaccines.