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Updated: Mar 25, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
Advances in Ene-Reductase Engineering: Dynamic Structure, Catalytic System Innovation, and Green Application
Mengya Qin1, Danni Yuan1, Qinglan Yao1
1State Key Laboratory for Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing 210037, China.
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Ene-reductases (ERs) are FMN- and NAD(P)H-dependent oxidoreductases that catalyze the stereoselective alkene double bond reduction. Recent advances in structural and synthetic biology have deepened our insights into their catalytic mechanisms and promoted molecular modification strategies. This review summarizes ERs' dynamic structural regulation, directed evolution, immobilization techniques, and applications in drug synthesis and environmental remediation. It highlights the impacts of conformational changes on catalytic selectivity, photoenzyme cooperative systems, and AI-assisted design, providing theoretical references for further research. Emerging promising approaches include low-cost immobilization, continuous-flow processes, and multifield coupling catalytic systems, while future research will focus on light-driven systems, coenzyme regeneration, non-natural radical reactions, and microbial cell factories.
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