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Development of an Efficient In Vitro Propagation Method for Acmella oleracea (L.) R.K. Jansen.

Pál Szarvas1, Judit Csabai2, Anzhela Kolesnyk3

  • 1Centre for Agricultural Genomics and Biotechnology, Faculty of the Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, H-4400 Nyíregyháza, Hungary.

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This study developed an efficient in vitro propagation protocol for Acmella oleracea using specific plant growth regulators and media. This standardized method supports research and industrial applications, particularly in cosmetics.

Keywords:
Acmella oleraceacarbon sourcecytokininsdisinfectionin vitro culturemicropropagationrootingshoot multiplicationtissue culture

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Area of Science:

  • Plant Biotechnology
  • Horticulture
  • In Vitro Culture

Background:

  • Acmella oleracea (L.) R.K. Jansen is a globally cultivated ornamental plant.
  • Lack of standardized in vitro propagation limits research and industrial use requiring uniform plant material.

Purpose of the Study:

  • To establish a standardized in vitro propagation protocol for Acmella oleracea.
  • To optimize media and plant growth regulator conditions for shoot multiplication and rooting.

Main Methods:

  • In vitro cultures established from seed germination; nodal segments used as explants.
  • Evaluated various Murashige and Skoog (MS) and McCown Woody Plant media formulations.
  • Tested different concentrations of saccharose and glucose as carbohydrate sources, and meta-Topolin and Benzyladenine as cytokinins.

Main Results:

  • Optimal shoot multiplication achieved with 13.2 µM meta-Topolin and 3% saccharose, or 13.2 µM Benzyladenine and 2% glucose.
  • In vitro rooting completed within 13 days.
  • Acclimatization of plantlets achieved within 8 days.

Conclusions:

  • A robust in vitro protocol for Acmella oleracea propagation has been developed.
  • This protocol provides standardized biomass essential for industrial applications, especially in the cosmetics sector.