Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

4.0K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
4.0K
Polarity of the Cytoskeleton01:18

Polarity of the Cytoskeleton

25.5K
The intrinsic polarity of cells can be primarily attributed to two factors- i) the asymmetric accumulation of mobile components such are regulatory molecules and subcellular components across the cell and ii) the orientation of polar cytoskeletal filaments that make up the cytoskeletal networks, specifically microfilaments, and microtubules arranged along the axis of polarity. Interactions between the cytoskeletal filaments are crucial for the establishment and maintenance of the polar nature...
25.5K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.7K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.7K
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

8.2K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.2K
Cell Migration01:09

Cell Migration

19.1K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
19.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromokinesin Klp-19 regulates microtubule overlap and dynamics during anaphase in C. elegans.

Journal of cell science·2026
Same author

Multivalent assembly of PAR-3/aPKC complexes establishes cell polarity in <i>Caenorhabditis elegans</i> zygotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Opposing actomyosin pools establish epithelial polarity during naive pluripotency exit.

The Journal of cell biology·2026
Same author

Targeting the cell membrane in established and emerging model organisms.

Development (Cambridge, England)·2026
Same author

A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function.

Nature communications·2025
Same author

Targeting the cell membrane in established and emerging model organisms.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Mar 27, 2026

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
10:03

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Published on: December 12, 2014

8.5K

Cell biology: Staying polarized without being antagonized.

Daniel J Dickinson1

  • 1Department of Molecular Biosciences, The University of Texas at Austin, NHB 2.606, Stop A5000, 100 East 24(th) Street, Austin, TX 78712, USA.

Current Biology : CB
|March 24, 2026
PubMed
Summary

Cell polarity relies on energy-intensive feedback. This study identifies two cooperating positive feedback mechanisms that stabilize cell polarity in the Caenorhabditis elegans zygote without mutual antagonism.

More Related Videos

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
06:03

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Published on: June 23, 2023

897
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy

Published on: October 3, 2017

14.4K

Related Experiment Videos

Last Updated: Mar 27, 2026

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
10:03

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Published on: December 12, 2014

8.5K
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
06:03

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Published on: June 23, 2023

897
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy

Published on: October 3, 2017

14.4K

Area of Science:

  • Cell biology
  • Developmental biology
  • Genetics

Background:

  • Stable cell polarity is crucial for cellular functions.
  • Maintaining asymmetric molecular localization requires significant cellular energy.
  • Feedback mechanisms are essential for regulating cell polarity.

Purpose of the Study:

  • To investigate the mechanisms stabilizing cell polarity.
  • To identify the positive feedback loops involved in polarity maintenance.
  • To understand how these mechanisms function in the absence of antagonism.

Main Methods:

  • Utilized the Caenorhabditis elegans zygote as a model system.
  • Employed genetic and live-imaging techniques.
  • Analyzed molecular localization and feedback dynamics.

Main Results:

  • Discovered two distinct positive feedback mechanisms.
  • Demonstrated that these mechanisms cooperate to stabilize polarity.
  • Showed polarity stabilization occurs independently of mutual antagonism.

Conclusions:

  • Two cooperating positive feedback loops are key to stable cell polarity.
  • These mechanisms provide robustness to polarity maintenance.
  • The findings offer insights into fundamental developmental processes.