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Updated: Mar 27, 2026

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Published on: December 21, 2016
[Mediation effect analysis on aromatic hydrocarbon receptors and DNA oxidative damage with embryonic arrest caused by
1Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
Abstract:
Objective: To investigate the association between polycyclic aromatic hydrocarbons (PAHs) exposure and embryonic arrest, and the proportion of mediating effects of aryl hydrocarbon receptor (AHR) mRNA expression and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels on this association. Methods: Using a 1∶2 matched case-control study, a total of 92 pregnant women diagnosed with embryonic arrest from April 2021 to April 2022 were selected as the case group, and 184 normal preganant women who voluntarily requested for abortion in the same hospital during the same period were usaed as the control group according to the match ratio of 1∶2. Abortion villus tissues were collected to detect the expression levels of PAH-DNA adducts, AHR mRNA expression and 8-OHdG levels. Conditional logistic regression was used to analyze the association of the three levels with embryonic arrest, and the mediating effects of AHR mRNA expression, and 8-OHdG levels were analyzed with R 4.3.3 software Mediation package. Results: The levels of PAH-DNA adducts, AHR mRNA expression, and 8-OHdG levels were higher in the embryonic arrest group than in the control group, and the differences were significant (all P<0.001). The results of mediating analysis showed that 8-OHdG levels partially mediated the association between PAHs exposure and embryonic arrest, with a mediating effect of 0.18 (95%CI:0.06-7.42), accounting for 16.07% of the total. And the expression of AHR mRNA mediated the levels of PAH-DNA adducts and 8-OHdG levels, with a mediation effect of 0.74 (95%CI:0.07-1.94), accounting for 27.11% of the total. Conclusion: PAHs exposure is a risk factor for embryonic arrest, which might cause DNA oxidative damage through increased AHR expression, further leading to embryonic arrest.
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