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Updated: Mar 27, 2026
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Development of a novel duplex recombinase polymerase amplification (DRPA) assay for rapid detection of
Chenyan Yuan1, Ting Zhang2, Juan Huo2
1Department of Laboratory Medicine, Zhongda Hospital Southeast University, Nanjing, China.
Abstract:
Carbapenem-Resistant Acinetobacter baumannii (CRAB) poses a major public health threat due to its multidrug resistance and high mortality rates, necessitating rapid and accurate diagnostic tools for timely intervention and infection control. This study developed and evaluated a novel detection method combining duplex recombinase polymerase amplification (DRPA) with a lateral flow strip (LFS) for the rapid visual identification of CRAB. The DRPA-LFS assay targets blaOXA-23 and blaOXA-51 genes, enabling isothermal amplification at 37 °C followed by a simple visual readout. The method demonstrated high sensitivity (102 CFU). Results can be obtained within 40 min without the need for complex instrumentation, making it suitable for resource-limited environments. the method offers a portable, user-friendly, and cost-effective solution for CRAB detection.
