Related Experiment Video

Updated: Jul 13, 2026

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

12.3K

Sequence-based comparative secretome analysis reveals conserved core effectors and host lineage-specific divergence

Noman Ali1, Nan Wu1, Mahinur S Akkaya1

  • 1School of Bioengineering, Dalian University of Technology, Dalian, China.

Frontiers in Plant Science
|March 25, 2026
PubMed

Insights

Powdery mildew fungi use secreted proteins for host colonization. This study analyzed secretomes across five genera, revealing conserved and diverse protein families, including novel effectors like EqCmu and EqPdt, crucial for plant-pathogen interactions.

Area of Science:

  • Plant Pathology
  • Mycology
  • Proteomics

Background:

  • Powdery mildew fungi are obligate biotrophs relying on secreted proteins for plant tissue colonization.
  • Understanding these secreted proteins is key to deciphering host-pathogen interactions and developing disease management strategies.

Purpose of the Study:

  • To comprehensively compare predicted secretomes from diverse powdery mildew genera.
  • To identify conserved and lineage-specific secreted proteins, including potential effector molecules.
  • To characterize the functional and structural properties of these secreted proteins.

Main Methods:

  • Standardized secretome prediction and filtering workflow across 26 isolates from five genera.
  • Orthogroup clustering, N-terminal motif screening, subcellular localization prediction, and functional annotation.
  • Homology searches against known powdery mildew effectors and proteome-wide searches for conserved non-canonical effectors.

Main Results:

  • Identified 7,545 secretome candidates assigned to 1,399 orthogroups, showing conserved and diversified components across genera.
  • Secretome candidates were generally short, with cysteine content correlating with apoplast localization.
  • Discovered conserved effector modules and identified EqCmu and EqPdt as broadly conserved non-canonical effectors with high sequence and structural conservation.

Conclusions:

  • The powdery mildew secretome exhibits both conserved core components and extensive genus- and isolate-specific diversification.
  • Specific N-terminal motifs and protein features are associated with different genera and subcellular localizations.
  • Identification of conserved non-canonical effectors provides new targets for understanding and controlling powdery mildew diseases.
Keywords:
effector proteinshost-associated divergenceorthogrouppowdery mildew fungisecretome prediction

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