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Updated: Mar 27, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

Digitally programmable microfluidic valving for autonomous, high-resolution continuous chromatographic purification.

Yi-Cheng Liao¹, Chih-Yi Huang^1,2, Yu-Chuan Tang¹

¹Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300044, Taiwan. jenhuang@mx.nthu.edu.tw.

|March 25, 2026

Summary

This summary is machine-generated.

This study introduces a microfluidic system with programmable valves for continuous protein purification. It achieves high purity and stability in multiple cycles, advancing automated bioseparation processes.

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A Microfluidic-based Hydrodynamic Trap for Single Particles

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Published on: January 21, 2011

Area of Science:

Biotechnology
Analytical Chemistry
Chemical Engineering

Background:

Continuous microscale purification demands precise fluid handling and temporal control.
Current chromatography systems offer limited programmability and coordination for purification cycles.

Purpose of the Study:

To develop a microfluidic continuous protein purification method with digitally programmable valves.
To enable logic-driven chromatography with precise temporal control and contamination-free fraction discrimination.

Main Methods:

Utilized digitally programmable inlet (ICV) and collection (CCV) valves for logic-driven chromatography.
Implemented sub-second buffer switching and deterministic routing across parallel affinity columns.
Employed temporal gating via CCV for real-time, profile-guided fraction selection.

Main Results:

Achieved 70-89% purity for GFP-His6 over ten continuous cycles with high stability.
Demonstrated compatibility with structurally sensitive biologics like His6-tagged TRAIL, preserving functional activity.
System architecture minimizes dead volume, prevents cross-contamination, and supports diverse chromatographic modes.

Conclusions:

The developed microfluidic system advances purification platforms toward autonomous, precision-controlled operations.
This method offers a broadly applicable analytical framework for microscale purification.
Supports development of next-generation bioseparation and continuous biomanufacturing technologies.