Related Experiment Video
Updated: Mar 27, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Innovative bioproduction of natural food preservative 3-hydroxypropionaldehyde by evolved Limosilactobacillus reuteri
Kyo-Jang-Kwan Noh1, Jung-Hyun Ju1, Sun-Yeon Heo1
1Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 56212, Republic of Korea.
Abstract:
Limosilactobacillus reuteri produces 3-hydroxypropionaldehyde (3-HPA), a compound with broad-spectrum antimicrobial activity and promise as a natural food preservative. Using adaptive laboratory evolution, we developed L. reuteri NK45, a strain with enhanced 3-HPA tolerance. Ascorbic acid supplementation mitigated 3-HPA-induced stress, allowing L. reuteri NK45 to maintain high viability and sustain 3-HPA production during glycerol bioconversion. Under established conditions (800 mM glycerol, 100 mM ascorbic acid, 1 mM cyanocobalamin, 30 °C, OD600 = 100, 2 h), L. reuteri NK45 achieved a record 3-HPA titer of 592.0 ± 3.8 mM. The product showed strong antimicrobial activity against foodborne and pathogenic microorganisms, with minimum inhibitory concentrations of 1-3 mM. These findings establish L. reuteri NK45 as an efficient and sustainable platform for food-grade 3-HPA production, highlighting its industrial potential to enhance food safety and shelf life through a natural bio-additive approach.
More Related Videos
Related Concept Videos
Production of Organic Acids
Production of Pharmaceuticals
Production of Antibiotics
Upstream Processing
Microbes in the Production of Fermented Foods
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

