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Updated: Mar 27, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
Published on: December 6, 2013
Genome-scale modeling for rational design of microbial cells and cocultures
Jiazi Peng1, Shichao Ren2, Siyu Han2
1Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China; Faculty of Synthetic Biology, Shenzhen University of Advanced Technology, Shenzhen, China; State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
None:
Microbial cell factories are powerful platforms for the sustainable production of chemicals, foods, medicines, and energy. Enhancing production efficiency requires a deep understanding of the underlying metabolic mechanisms. Over the past 3 decades, genome-scale models have enabled quantitative metabolic simulation and driven the development of algorithms for guiding rational metabolic engineering. In this review, we provide an overview of recent algorithms for microbial cell design and the most comprehensive evolutionary roadmap to date, emphasizing their development and implementation. We also summarize advances in modeling frameworks and algorithms for coculture design, compare algorithms for microbial cells and cocultures, and discuss emerging trends shaping future model and algorithm development.
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