In vitro platforms for reconstructing skin-microbiome interactions

The growing recognition of the skin microbiome in regulating the host's metabolic and immune functions has spurred the development of in vitro platforms designed to recapitulate their intricate interactions, aiding research into both skin-microbiome and microbe-microbe interactions within their distinct niches. Despite these efforts, challenges remain in dissecting skin-microbiome interactions, especially due to the absence of a standardized platform for the long-term coculture of bacterial and mammalian cells. In this review, we highlight the key components in modeling an in vitro skin ecosystem and discuss the therapeutic potential of skin commensals, including recent advances and applications for engineered live biotherapeutics targeting skin diseases, to underscore the translational value of in vitro skin-microbiome interaction studies.