Integrated GC-MS, LC-MS/MS metabolomics, and transcriptomics analysis of meat quality differences between marketed
1Key Laboratory of Tropical Animal Breeding and Epidemic Disease Research, Institute of Animal Husbandry and Veterinary Research, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
Abstract:
Targeting China' s pork market transformation and high-end sector bottlenecks, this study employed 6 WZS pigs (experimental group) and 6 DLY pigs (control group), aged 7-8 months. After slaughter, longissimus thoracis samples were collected for meat quality and molecular mechanism analysis using multiple technical methods. The results showed that compared with DLY pigs, (1) they exhibited distinct meat quality advantages, including higher muscle fiber density, a larger proportion of Type II A muscle fibers, and higher L* and a* values (P < 0.01), as well as lower drip loss and shear force (P < 0.05); (2) their pork contained more abundant sulfur-containing volatile substances, with superior umami, saltiness, and lower astringency. Omics analyses identified 12 DFMs, 361 DMs, and 2279 DEGs, these substances induce differences in meat quality by regulating relevant pathways. Integrated multi-omics found 46 core regulators. WZS pigs excel in flavor and tenderness, and China' s pork industry development.
