Protocol to isolate, expand, and activate human type 2 innate lymphoid cells to study immune checkpoint function

Type 2 innate lymphoid cells (ILC2s) are a subset of innate lymphoid cells involved in allergic diseases, tissue repair, and tumor immunity. Here, we present a protocol to study the function of immune checkpoint receptors on ILC2s in vitro using plate-bound recombinant ligands. We describe steps for the enrichment, expansion, and purification of human ILC2s from healthy donors' peripheral blood. For complete details on the use and execution of this protocol, please refer to Ciancaglini et al.¹.