Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifunctional-engineered NK cells overcome tumor immunosuppression by combining PD-L1 and HLA-E targeting and endogenous IL15 production.

Signal transduction and targeted therapy·2026
Same author

HLA-G expression in non-small cell lung cancer: prognostic significance and interplay with PD-L1 and CD8<sup>+</sup> tumor-infiltrating lymphocytes.

Frontiers in immunology·2026
Same author

Autophagy supports the storage of lytic granules in human NK cells.

Cell death & disease·2026
Same author

The transmembrane IL-15 isoform expressed on human melanoma cells triggers modulatory effects on tumor progression upon stimulation with the soluble IL-15Rα chain.

Frontiers in immunology·2026
Same author

ILC2s and their immune checkpoints in the antitumor response.

Journal for immunotherapy of cancer·2026
Same author

Immune correlates underlying small fiber neuropathy presenting as vaccine-associated post-acute SARS- coronavirus syndrome.

Frontiers in immunology·2026

Related Experiment Video

Updated: Mar 28, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

2.7K

Protocol to isolate, expand, and activate human type 2 innate lymphoid cells to study immune checkpoint function.

Cecilia Ciancaglini1, Silvia Santopolo1, Paola Vacca1

  • 1IRCCS Bambino Gesù Children's Hospital, Innate Lymphoid Cell Unit, Rome, Italy.

STAR Protocols
|March 26, 2026
PubMed
Summary

This study details a method to investigate immune checkpoint receptors on type 2 innate lymphoid cells (ILC2s). The protocol enables researchers to explore ILC2 functions in allergic diseases and immunity.

Keywords:
CancerCell BiologyCell cultureCell isolationFlow CytometryImmunology

More Related Videos

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches
08:25

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

14.4K
Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

22.1K

Related Experiment Videos

Last Updated: Mar 28, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

2.7K
Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches
08:25

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

14.4K
Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

22.1K

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Type 2 innate lymphoid cells (ILC2s) are crucial immune cells involved in allergic responses, tissue repair, and tumor immunity.
  • Understanding ILC2 function is vital for developing new therapeutic strategies.

Purpose of the Study:

  • To present a detailed protocol for studying immune checkpoint receptor function on human ILC2s in vitro.
  • To provide a method for the enrichment, expansion, and purification of ILC2s for experimental use.

Main Methods:

  • Isolation and purification of human ILC2s from peripheral blood of healthy donors.
  • In vitro culture of ILC2s.
  • Stimulation of ILC2s using plate-bound recombinant ligands to study immune checkpoint receptors.

Main Results:

  • The protocol successfully enables the study of immune checkpoint receptor function on ILC2s.
  • Researchers can now investigate the role of these receptors in ILC2-mediated processes.

Conclusions:

  • This protocol offers a valuable tool for the immunological research community.
  • It facilitates further investigation into the complex roles of ILC2s in health and disease.