Application of near-infrared spectroscopy for the classification of crambe seeds according to physiological potential

Near-infrared (NIR) spectroscopy has emerged as a promising tool for saving time and resources in both industrial and seed testing laboratories, as it enables the rapid pre selection of seed lots without the need for chemical reagents or the generation of waste. The application of this technique allows for the fast and accurate discrimination of seed lots according to their physiological potential. This study aimed to assess the feasibility of using the NIR technique, in combination with traditional physiological tests, to detect differences and classify crambe seed lots based on their physiological potential. Seeds from four crambe lines, including original (non-aged) seed lot and those artificially aged at 42 °C for 48 and 96 h, were evaluated. Germination and vigor of both seeds and seedlings were assessed according to standardized physiological tests. NIR spectra were collected from intact seed samples. Artificial aging induced deterioration at varying levels among the lines, particularly in the seed lot aged for 96 h, revealing differences in seed physiological potential. The spectral region between 1300 and 1400 nm was identified as the most relevant for distinguishing physiological potential levels in crambe seeds. The wavelengths that contributed most to classifying the seed lots into different levels of physiological potential were associated with biochemical compounds essential for seed quality. Therefore, near-infrared spectroscopy demonstrates strong potential for use in classifying crambe seeds according to their physiological potential.