Generation and characterization of a human induced pluripotent stem cell line (hiPSC) expressing the rEstus voltage
Daniela Malan1, Philipp Rühl2, Stefan H Heinemann2
1Institute of Physiology I, Medical Faculty, University of Bonn, Bonn, Germany.
Abstract:
The UKBi015-B-6 human induced pluripotent stem cell (hiPSC) line was generated by targeted insertion of a doxycycline-inducible TRE3G expression cassette encoding the ultrasensitive genetically encoded fluorescent voltage indicator rEstus into the AAVS1 locus. This hiPSC line enables controlled, reversible induction of rEstus expression to non-invasively monitor membrane potential dynamics in a human cellular background. The resulting platform allows high-throughput optical electrophysiology across diverse hiPSC-derived lineages, including cardiomyocytes, neurons, and non-excitable cells, thereby facilitating drug screening, developmental studies, and quantitative analysis of bioelectrical signaling under standardized, human-relevant conditions.
