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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Alcohol Dehydrogenase-Mediated Generation of Cytotoxic Thiophene-Containing Eremophilane Sesquiterpenes
Hengyi Xu1, Xiaonan Deng1, Yawen Zhang1
1Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Abstract:
Sulfur-containing heterocyclic natural products are valued for their bioactivity and unique biosynthesis. By reconstituting the jan cluster derived from the marine fungus Penicillium janthinellum H7-4, we discovered two thiophene-bearing cytotoxic eremophilanes, janthiophenes A and B (1 and 2), together with ten unreported congeners. Biosynthetic studies demonstrated that JanH, a key alcohol dehydrogenase, is responsible for the formation of the janthiophene structures. These findings enrich the oxidoreductase toolbox for the biosynthesis of sulfur-containing architectures.
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