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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
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Biocatalysis Under Reduced Pressure; Two-Step, One-Pot Amide Synthesis Using an Immobilised Transaminase/Lipase
Lisa Kennedy1, Nicholas Mulholland2, Andrew Gomm2
1School of Chemistry, University of Edinburgh, Edinburgh, UK.
Chembiochem : a European Journal of Chemical Biology
|March 27, 2026
Summary
Immobilized enzymes enable efficient biocatalysis. A novel rotary evaporator method coupled with a lipase and transaminase cascade reaction achieves high yield and enantiopurity for organic synthesis.
Area of Science:
- Biocatalysis and organic synthesis
- Enzyme immobilization techniques
Background:
- Enzyme immobilization enhances biocatalyst stability and reusability in non-conventional conditions.
- Biocatalytic cascades offer advantages but face challenges like enzyme/substrate incompatibility and long reaction times.
Purpose of the Study:
- To develop an innovative method for lipase-catalyzed reactions using a rotary evaporator under reduced pressure.
- To establish a one-pot, two-step biocatalytic cascade combining lipase and transaminase for efficient organic synthesis.
Main Methods:
- Utilized a rotary evaporator for in situ by-product removal, shifting reaction equilibrium.
- Developed a cascade by coupling immobilized lipase with a pyridoxal 5'-phosphate (PLP)-dependent transaminase (ATA).
- Optimized immobilization support, solvent, and water content for enzyme compatibility.
Main Results:
- Achieved high enantiopurity in the kinetic resolution of a chiral amine using immobilized lipase.
- Demonstrated a highly efficient enantioselective ATA/lipase cascade converting a prochiral ketone to an amide.
- Obtained high yield and >99% enantiomeric excess (%ee) in the cascade reaction.
Conclusions:
- The rotary evaporator method facilitates biocatalytic reactions by managing volatile by-products.
- The developed one-pot cascade reaction efficiently synthesizes chiral amides with high enantioselectivity.
- This approach simplifies the combination of biocatalysts for organic synthesis using standard laboratory equipment.
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