Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

24.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
The Tumor Microenvironment02:17

The Tumor Microenvironment

8.1K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Honokiol and Its Emerging Role in Breast Cancer Therapy.

Cancers·2026
Same author

Distinct immune responses to HIV and CMV in Hofbauer cells across gestation highlight evolving placental immune dynamics.

Frontiers in immunology·2026
Same author

CircRNA profiles of extracellular vesicle-enriched fractions from ART suppressed pregnant women living with HIV identifies interactome networks key to inflammation and viral latency.

Journal of translational medicine·2026
Same author

Building Capacity for Rigorous Health Research Through Grant Writing Coaching.

International journal of environmental research and public health·2026
Same author

Placental biology links genetic, epigenetic, ancestral, and social determinants to maternal-fetal health inequities.

Frontiers in reproductive health·2026
Same author

Inhibition of CD154:CD11b interactions using a novel nanotherapeutic improves allograft survival.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same journal

RETRACTED: Bakshi et al. Crocin Inhibits Angiogenesis and Metastasis in Colon Cancer via TNF-α/NF-kB/VEGF Pathways. <i>Cells</i> 2022, <i>11</i>, 1502.

Cells·2026
Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026
Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026
Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026
Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026
Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.8K

SMR Peptide Modulates Tumor-Derived Extracellular Vesicles microRNA and Inflammatory Transcript Signatures in TNBC.

Ming-Bo Huang1, Fengxia Yan2, Uswa Jadoon1

  • 1Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, GA 30310, USA.

Cells
|March 27, 2026
PubMed
Summary
This summary is machine-generated.

The synthetic SMRwt peptide restores tumor-suppressive microRNAs (miRNAs) and suppresses inflammasome signaling in triple-negative breast cancer (TNBC). This dual action supports SMRwt as a potential therapeutic strategy for TNBC progression.

Keywords:
NLRP3SMR peptideTNBCapoptosis-associated speck-like protein (ASC)caspase-1exosomesextracellular vesicles (EVs)inflammasomemicroRNA (miRNA)tumor-derived EVs (tEVs)

More Related Videos

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
08:12

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

Published on: June 12, 2019

5.5K
Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.3K

Related Experiment Videos

Last Updated: Mar 29, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.8K
The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
08:12

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

Published on: June 12, 2019

5.5K
Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.3K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Triple-negative breast cancer (TNBC) is aggressive, lacks targeted therapies, and shows miRNA dysregulation linked to epithelial-to-mesenchymal transition (EMT) and metastasis.
  • Tumor-derived extracellular vesicles (tEVs) promote TNBC via oncogenic cargo and pro-inflammatory signaling.
  • The synthetic SMRwt peptide's effects on TNBC EV miRNA composition and inflammatory profiles are largely unknown.

Purpose of the Study:

  • To investigate if SMRwt peptide alters tEV-associated miRNAs and cytokine transcript signatures relevant to EMT and inflammasome pathways in TNBC.
  • To assess SMRwt's impact on miRNA networks and inflammatory signaling in TNBC.
  • To explore SMRwt's potential as a dual-target therapeutic strategy.

Main Methods:

  • Isolated extracellular vesicles (EVs) from SMRwt-treated and untreated MDA-MB-231 TNBC cells.
  • Performed nanoparticle tracking analysis and small RNA sequencing on isolated EVs.
  • Utilized NanoString nCounter Breast Cancer 360 Gene Expression Panel for transcriptomic profiling.

Main Results:

  • SMRwt treatment enriched 11 tumor-suppressive miRNAs (e.g., Let-7a-5p, miR-26b-5p) associated with reduced proliferation, EMT, migration, and metastasis.
  • Observed non-significant decreases in oncogenic miRNAs (e.g., miR-1200, miR-374a-5p) implicated in cancer progression.
  • Demonstrated reduced expression of inflammasome-associated cytokines (e.g., IL-1β) and suppressed ASC-mediated caspase-1 activation and IL-1β secretion, inhibiting NLRP3 inflammasome signaling.

Conclusions:

  • SMRwt peptide restores tumor-suppressive miRNA networks in TNBC.
  • SMRwt suppresses inflammasome-driven inflammation by inhibiting NLRP3 signaling.
  • SMRwt exhibits potential as a dual-target therapeutic strategy for TNBC by targeting both miRNA dysregulation and inflammation.