Related Experiment Video

Updated: Mar 29, 2026

Studying Chronic Exposure of Mice to Ultraviolet B Radiation
03:20

Studying Chronic Exposure of Mice to Ultraviolet B Radiation

Published on: August 19, 2025

2.2K

Isaridin E Protects Against UVB-Induced Photoaging by Activating Wnt/β-Catenin Signaling Pathway and Alleviating

Yaosheng Liu1,2, Weizhen Li1,3, Zeen Yang1

  • 1Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, China.

Marine Drugs
|March 27, 2026
PubMed

Insights

Isaridin E (ISE) combats skin photoaging by improving mitochondrial function and activating the Wnt/β-catenin pathway. This natural compound reduces cellular senescence and restores skin health, offering a potential therapeutic strategy.

Area of Science:

  • Dermatology
  • Cell Biology
  • Molecular Biology

Background:

  • Mitochondrial dysfunction significantly contributes to skin photoaging.
  • The Wnt/β-catenin pathway is crucial for regulating cellular processes and can ameliorate mitochondrial abnormalities.
  • Targeting the Wnt/β-catenin pathway presents a therapeutic avenue for photoaging.

Purpose of the Study:

  • To investigate the anti-senescence effects of Isaridin E (ISE) on skin fibroblasts affected by photoaging.
  • To elucidate the role of the Wnt/β-catenin pathway in ISE's anti-photoaging mechanisms.

Main Methods:

  • In vitro studies using UVB-exposed fibroblasts to assess cell viability, senescence markers (β-galactosidase), and senescence-associated secretory phenotype (SASP) factors.
  • In vivo studies utilizing a photoaging mouse model to evaluate skin thickness, collagen expression, and tissue SASP levels.
  • RNA sequencing and Western blotting to analyze Wnt/β-catenin signaling pathway activity.
  • GSK-3β-targeted siRNA to confirm pathway mediation.

Main Results:

  • ISE dose-dependently improved UVB-induced fibroblast damage, reduced senescence markers, and suppressed SASP production.
  • In vivo, ISE decreased skin thickness, enhanced dermal collagen, and lowered tissue SASP levels.
  • ISE significantly ameliorated mitochondrial dysfunction and energy deficits in fibroblasts.
  • UVB irradiation suppressed Wnt/β-catenin signaling, which ISE restored dose-dependently.
  • ISE's anti-photoaging effects were confirmed to be mediated through the Wnt/β-catenin pathway.

Conclusions:

  • Isaridin E (ISE) demonstrates significant anti-photoaging properties by mitigating UVB-induced skin damage and cellular senescence.
  • ISE enhances Wnt/β-catenin pathway activity and improves mitochondrial function, counteracting key aspects of photoaging.
  • ISE represents a promising marine-derived natural product for therapeutic intervention in skin photoaging.
Keywords:
Isaridin EWnt/β-cateninmitochondrial dysfunctionphotoagingultraviolet B

More Related Videos

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract13:56

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Published on: November 28, 2012

10.5K
Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium06:56

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Published on: March 14, 2015

10.5K

Related Concept Videos

Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
45.5K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.5K
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
21.3K
Acne Infection01:27

Acne Infection

Acne is a multifactorial skin condition primarily affecting adolescents and young adults, with a global prevalence estimated to exceed 75% in this demographic. The condition is characterized by the formation of comedones (blackheads and whiteheads), papules, pustules, nodules, and, in severe cases, cysts, particularly in areas rich in sebaceous glands such as the face, neck, chest, and back. The pathogenesis involves increased sebum production, follicular hyperkeratinization, colonization by...
43
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.7K