In Vitro Biocompatibility of Calcium Silicate-Based Materials for Retrograde Endodontic Treatment Under Different...

Area of Science:

• Biomaterials Science
• Dental Materials
• Cell Biology

Background:

• Calcium silicate-based materials are vital for endodontic treatments due to their bioactivity.
• Setting environment and component release influence cellular responses, requiring further investigation.

Purpose of the Study:

• Evaluate cellular response to MTA+, Biodentine, and NeoPUTTY after setting in varied environments.
• Assess biocompatibility based on eluate-mediated effects on human fibroblasts.

Main Methods:

• Materials set in dry, PBS, or blood environments.
• Eluates collected at 1, 3, and 5 days.
• Human BJ fibroblasts exposed to eluates; cell viability assessed via AlamarBlue assay.

Main Results:

• Setting environment and eluate age significantly affected cell viability.
• Biodentine showed the most stable cellular response across conditions.
• Blood setting minimally impacted cell viability compared to PBS and dry conditions.

Conclusions:

• Blood exposure during setting had minimal influence on tested materials' cell viability.
• Biodentine demonstrated the most consistent biological profile.
• Findings highlight time-dependent, eluate-mediated effects, requiring cautious clinical interpretation.