Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

2.5K
2.5K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

4.0K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
4.0K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.3K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.3K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

9.3K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
9.3K
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

113.4K
Overview
113.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nucleolin in the cell membrane promotes Ang II-mediated VSMC phenotypic switching by regulating the AT1R internalization function : Nucleolin promotes Ang II-mediated VSMC phenotypic switching.

Biology direct·2025
Same author

Identification of lncRNAs associated with the pathogenesis of ankylosing spondylitis.

BMC musculoskeletal disorders·2021
Same author

Interleukin-17 activates and synergizes with the notch signaling pathway in the progression of pancreatic ductal adenocarcinoma.

Cancer letters·2021
Same author

Association of sleep disorders with subfoveal choroidal thickness in preschool children.

Eye (London, England)·2021
Same author

Omega-3 fatty acids protect from colitis via an Alox15-derived eicosanoid.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2021
Same author

Oxidative phosphorylation enhances the leukemogenic capacity and resistance to chemotherapy of B cell acute lymphoblastic leukemia.

Science advances·2021
Same journal

Optimized flow cytometry assay for functional characterization of variants of uncertain significance in familial hypercholesterolemia.

Atherosclerosis·2026
Same journal

Causal insights of modifiable cardiovascular risk factors for dementia risk - potential for efficient prevention and improved brain health.

Atherosclerosis·2026
Same journal

Serial OCT-based coronary physiology and plaque composition in vessels with nonobstructive coronary lesions following intensive lipid-lowering therapy: YELLOW III sub-study.

Atherosclerosis·2026
Same journal

Earliest age to detect lifetime cardiometabolic health stratification in children.

Atherosclerosis·2026
Same journal

Sympathetic neurons exacerbate atherosclerosis by modulating macrophage function via the NPY/Y1R axis.

Atherosclerosis·2026
Same journal

Optimizing lipoprotein(a) testing for immediate clinical impact in primary prevention.

Atherosclerosis·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein
07:26

Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein

Published on: August 12, 2025

819

Cell surface nucleolin promotes endothelial cell pyroptosis in atherosclerosis through RASSF2.

Li Fang1, Zhijie Shen1, Dan Huang1

  • 1Cardiovascular Department Second Ward, The Affiliated Changsha Hospital of Xiangya School of Medicine, Central South University, Changsha, China.

Atherosclerosis
|March 27, 2026
PubMed
Summary
This summary is machine-generated.

Nucleolin (NCL) promotes atherosclerosis by increasing endothelial cell pyroptosis. Targeting NCL may offer a new therapeutic strategy for treating this cardiovascular disease.

Keywords:
AtherosclerosisNucleolinPyroptosisRASSF2

More Related Videos

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology
05:51

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

Published on: May 6, 2014

13.8K
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
06:09

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Published on: August 20, 2019

17.4K

Related Experiment Videos

Last Updated: Mar 29, 2026

Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein
07:26

Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein

Published on: August 12, 2025

819
A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology
05:51

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

Published on: May 6, 2014

13.8K
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
06:09

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Published on: August 20, 2019

17.4K

Area of Science:

  • Cardiovascular Biology
  • Cellular Mechanisms of Disease
  • Molecular Pathology

Background:

  • Atherosclerosis (AS) progression is linked to pyroptosis in endothelial cells (ECs).
  • Nucleolin (NCL) is implicated in vascular smooth muscle cell proliferation in AS.
  • The role of cell surface NCL in mediating EC pyroptosis during AS is not well understood.

Purpose of the Study:

  • To investigate the mechanism by which cell surface NCL mediates pyroptosis in ECs during AS.
  • To explore NCL's role in AS pathogenesis and identify potential therapeutic targets.

Main Methods:

  • Induction of AS in ApoE-/- mice fed a high-fat diet.
  • Assessment of aortic lesions, EC pyroptosis, inflammation, and NCL expression.
  • Investigation of NLRP3 inflammasome inhibition and NCL modulation effects.
  • Mechanistic studies using immunoprecipitation-mass spectrometry (IP-MS).

Main Results:

  • AS mice showed severe aortic lesions, increased EC pyroptosis, inflammation, and NCL expression.
  • NLRP3 inhibition and NCL knockdown reduced AS severity and EC pyroptosis.
  • NCL interacted with RASSF2, facilitating its nuclear translocation and exacerbating EC pyroptosis and inflammation.

Conclusions:

  • NCL promotes AS progression by enhancing EC pyroptosis via RASSF2 nuclear transport.
  • This study elucidates NCL's mechanistic role in AS, identifying a novel pathway and potential therapeutic targets.