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Mechanistic Insights into Active Components of Rosa Roxburghii Juice Against Fluoride-Induced Osteoarthritis
Youqi Du1,2,3,4, Youwen Du1,2,3,4, Shaobo Liu1,2,3,4
1The State Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmaceutical Sciences Guizhou Medical University, No. 6 Ankang Avenue, Guiyang 561113, China.
Antioxidants (Basel, Switzerland)
|March 28, 2026
Summary
Rosa roxburghii juice protects against fluoride-induced osteoarthritis (F-OA) by targeting the TP53 pathway. Key compounds like quercetin and epicatechin attenuate cellular injury and restore protein balance, offering a promising functional food for F-OA prevention.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- Fluoride-induced osteoarthritis (F-OA) is a severe condition linked to endemic fluorosis with few treatment options.
- Rosa roxburghii juice (RRJ) shows promise for skeletal fluorosis, but its active components and mechanisms remain unclear.
Purpose of the Study:
- To elucidate the protective mechanisms of RRJ against F-OA using an integrated multi-omics approach.
- To identify key bioactive compounds in RRJ and their molecular targets involved in F-OA.
Main Methods:
- Bioinformatics, network pharmacology, molecular docking, molecular dynamics simulations, limited proteolysis-mass spectrometry (LiP-MS), and in vitro cell experiments.
- Identification of 44 core F-OA genes, highlighting TP53 and its signaling pathway.
- Screening of key RRJ compounds including quercetin, epicatechin, emodin, and ellagic acid.
Main Results:
- Quercetin, epicatechin, emodin, and ellagic acid showed strong binding affinities to F-OA targets, particularly TP53.
- These compounds effectively reduced sodium fluoride-induced cellular injury in vitro.
- LiP-MS revealed protein structural changes, with TP53 showing significant sensitivity; compounds suppressed p53 mRNA and enhanced proteasomal degradation.
Conclusions:
- RRJ's active compounds protect against F-OA by modulating the p53 pathway, suppressing p53 mRNA, and restoring proteasome-mediated degradation.
- Rosa roxburghii fruit represents a potential functional food for preventing and managing skeletal fluorosis and F-OA.
