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Updated: Mar 29, 2026
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Published on: November 28, 2016
A DPO-Enhanced Gold Nanoparticle-Assisted PCR Assay for Simultaneous Detection of Bovine Viral Diarrhea Virus,
Dongjie Cai1, Yulin Lin1, Jie Chen2
1Key Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Abstract:
To develop a rapid detection tool for major bovine diarrheal viruses, Bovine viral diarrhea virus (BVDV), Bovine astrovirus (BAstV), and Bovine rotavirus (BRV), a gold nanoparticle-enhanced dual-priming oligonucleotide (DPO) multiplex PCR method was established. After optimization, the assay showed high sensitivity with detection limits of 4.9 × 10-1, 2.72 × 102, and 1.88 × 103 copies/μL for BVDV, BAstV, and BRV, respectively, and demonstrated excellent specificity. In the validation using 963 clinical samples, the DPO-nanoPCR showed numerically higher detection rates for BVDV (5.50% vs. 4.36%) and BAstV (1.04% vs. 0.62%) compared to conventional PCR, while the detection rate for BRV was identical (0.93%). Statistical analysis indicated no significant difference between the two methods (p > 0.05), with an overall agreement rate exceeding 98.86%. The developed DPO-nanoPCR method provides a sensitive, specific, and efficient tool for the simultaneous surveillance and diagnosis of these key bovine enteric pathogens.
