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UV-Activated Zwitterionic Fluorine-Containing Hybrid Probe for Dual-Modal 19F/1H MRI.

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Researchers developed a water-soluble dual-modal magnetic resonance imaging (MRI) probe to overcome aggregation issues. This new probe uses UV light to activate its imaging signals, improving performance for enhanced dual-mode imaging.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Radiology

Background:

  • Dual-modal 19F/1H MRI probes face challenges with hydrophobic aggregation due to high fluorine content, impacting solubility and imaging capabilities.
  • Developing water-soluble probes is crucial for improving the performance and applicability of MRI contrast agents.

Purpose of the Study:

  • To design and synthesize a water-soluble, activatable dual-modal 19F/1H MRI probe.
  • To investigate the UV-triggered paramagnetic relaxation enhancement (PRE) modulation for activatable MRI contrast.
  • To evaluate the probe's imaging performance, solubility, biocompatibility, and cellular uptake.

Main Methods:

  • RAFT polymerization and ring-opening reactions were employed to graft fluorinated phosphocholine zwitterions and Gd3+ chelates onto a UV-responsive polymer.
  • The synthesized probe (PFCP-Gd) was characterized for its water solubility and dual-modal MRI properties.
  • Paramagnetic relaxation enhancement (PRE) modulation was studied under UV irradiation.
  • In vitro experiments assessed biocompatibility and cellular uptake efficiency.

Main Results:

  • A water-soluble dual-modal probe (PFCP-Gd) was successfully synthesized.
  • UV irradiation triggered PRE modulation, enabling activatable 19F/1H MRI contrast.
  • Upon UV irradiation, PFCP-Gd showed a significantly enhanced 19F signal (T2 relaxation time of 23 ms, SNR of 11.34 at 12 mM 19F).
  • The probe demonstrated good biocompatibility and efficient cellular uptake.

Conclusions:

  • The developed PFCP-Gd probe offers a strategy for constructing water-soluble, activatable fluorinated MRI probes.
  • This approach addresses the aggregation issue in dual-modal MRI probes, enhancing their imaging performance.
  • The activatable nature of the probe allows for controlled and improved dual-mode imaging applications.