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Updated: Mar 30, 2026
Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
Published on: September 30, 2014
Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in
Dominique Engel1, Laurent Nguyen2
1Laboratory of Molecular Regulation of Neurogenesis, GIGA Institute, University of Liège, 4000 Liège, Belgium.
Abstract:
We present a protocol for whole-cell recordings from hippocampal CA2 pyramidal neurons in acute adult mouse brain slices. We describe the preparation of viable dorsal hippocampal slices, the visual identification and recording of CA2 neurons, and intracellular labeling. We then detail post hoc immunohistochemical verification using CA2-specific markers. This approach enables reliable functional characterization of CA2 neurons and unambiguous confirmation of their anatomical localization. For complete details on the use and execution of this protocol, please refer to Le Bail et al.1.
