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Updated: Mar 31, 2026
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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
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Advances in Dehalogenase Biocatalysis: Mechanisms, Engineering, and Industrial Perspectives
Jinxiu Wang1,2, Yao Zhang1,2, Mengqi Li1,2
1State Key Laboratory of Microbial Technology, College of Life Science, Nanjing Normal University, No. 1 Wenyuan Road, Nanjing 210097, China.
Journal of Agricultural and Food Chemistry
|March 30, 2026
Summary
Dehalogenase enzymes offer powerful solutions for environmental cleanup and green chemistry by efficiently removing harmful halogenated compounds. Advances in enzyme engineering and AI design are enhancing their capabilities for bioremediation and synthesis.
Area of Science:
- Biochemistry
- Environmental Science
- Biotechnology
Background:
- Halogenated organic compounds pose environmental risks due to their persistence.
- Dehalogenases are enzymes that can remove halogens, offering potential for remediation and synthesis.
- Examples include LinB and HHDH enzymes with specific catalytic functions.
Purpose of the Study:
- To review current advances in dehalogenase research.
- To highlight enzyme engineering and AI-assisted design in dehalogenase development.
- To discuss the application of dehalogenases in bioremediation and green chemistry.
Main Methods:
- Review of current literature on dehalogenase evolution, mechanisms, and classification.
- Analysis of recent enzyme engineering strategies.
- Exploration of AI-assisted design approaches for dehalogenases.
Main Results:
- Dehalogenases have diverse evolutionary origins and catalytic mechanisms.
- Enzyme engineering and AI design are improving dehalogenase stability, substrate scope, and function.
- Engineered dehalogenases show promise for environmental remediation and green synthesis.
Conclusions:
- Dehalogenases are evolving into efficient biocatalysts.
- These enzymes offer powerful tools for addressing environmental hazards and advancing green chemistry.
- Future applications in bioremediation and synthesis are significant.
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