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Area of Science:

  • Plant Pathology
  • Molecular Plant-Microbe Interactions
  • Plant Immunity

Background:

  • Non-host pathogens induce broad-spectrum plant resistance.
  • Syringolin A activates the defense gene Pir7b in rice, but the mechanism is unclear.

Purpose of the Study:

  • Investigate the role of OsNAC78 in the plant immune pathway.
  • Elucidate the mechanism of syringolin A-induced rice immunity.

Main Methods:

  • Transgenic rice lines (OsNAC78 overexpression/knockout, Pir7b overexpression/knockout) were created.
  • Plants were inoculated with Magnaporthe oryzae.
  • Promoter activity and gene expression were analyzed under syringolin A treatment.

Main Results:

  • OsNAC78 acts as a positive regulator of blast resistance.
  • Pir7b confers blast resistance.
  • Syringolin A activates the OsNAC78-Pir7b module, enhancing promoter activity and inducing Pir7b transcription.
  • OsNAC78 is essential for Pir7b expression and syringolin A-induced immunity.
  • Reactive oxygen species (ROS) accumulation is involved in the induced resistance.

Conclusions:

  • Syringolin A induces rice immunity via the OsNAC78-Pir7b pathway.
  • OsNAC78 regulates Pir7b expression, triggering blast resistance.
  • This study reveals a non-host pathogen-induced immune signaling mechanism in rice.