Related Experiment Video
Updated: Mar 18, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
OsCPN10a, cooperating with OsCPN20 and OsHSP60-3B negatively regulate ABA signaling and enhance seed storability in
Sufeng Liao1,2,3,4, Yidong Wei1,3,4, Yongsheng Zhu1,3,4
1Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China.
Abstract:
Seed storability is crucial for maintaining vigor at the sowing stage and ensuring agricultural sustainability. Although molecular chaperones are well characterized in protein homeostasis, their roles in regulating ABA receptor stability and starch integrity during seed aging remain unclear. Here, we demonstrate that OsCPN10a enhances storability by maintaining ABA homeostasis, suppressing ROS accumulation, and preserving starch granule structure. Genetic and biochemical analyses reveal that OsCPN10a functions upstream of the OsCPN20-OsHSP60-3B cascade, directly interacts with both partners to promote complex assembly, and negatively regulates ABA signaling. The resulting trimeric complex interacts with OsPYL10, facilitating receptor dissociation and high-affinity association with the PP2C phosphatase OsABIL1 to attenuate signaling. This study reveals a non-canonical function of molecular chaperones in directly modulating hormone receptor signaling, establishing a mechanistic link between protein homeostasis and ABA sensitivity in seeds.
More Related Videos
07:43Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
Related Concept Videos
Gene Regulation During Sporulation
Global Regulatory Systems
Cell Signaling in Plants
Adaptations that Reduce Water Loss