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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
101.0K
RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Updated: Mar 31, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Digital Image Segmentation Analysis Enables High-Throughput Next-Generation Impactor Analysis.

Jaston McClure1, Daniel Dobson1, Ed Yost1

  • 1Synthetic Molecule Pharmaceutical Science, Genentech, South San Francisco, California, USA.

Journal of Aerosol Medicine and Pulmonary Drug Delivery
|March 30, 2026
PubMed
Summary
This summary is machine-generated.

A new image analysis method offers faster aerodynamic particle size distribution (APSD) determination for inhaled therapeutics, providing results comparable to traditional HPLC methods.

Keywords:
APSD determinationNGINGI sample preparationhigh-throughput NGI analysis

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Area of Science:

  • Pharmaceutical technology
  • Analytical chemistry
  • Biomedical engineering

Background:

  • Aerodynamic particle size distribution (APSD) is critical for inhaled therapeutics.
  • Current gold standard methods like the next-generation impactor (NGI) require lengthy HPLC analysis.
  • There is a need for faster, efficient APSD determination techniques.

Purpose of the Study:

  • To develop and validate a novel image analysis method for APSD determination.
  • To reduce the analysis time associated with traditional APSD measurement techniques.
  • To provide a faster alternative for quality control of inhaled dry powder formulations.

Main Methods:

  • Development of an image analysis technique for APSD measurement.
  • Application of the method to neat active pharmaceutical ingredient (API), API/lactose blends, and stressed samples.
  • Comparison of image analysis results with traditional HPLC-derived data.

Main Results:

  • The image analysis method demonstrated sufficient resolution for differentiating particle sizes.
  • The technique successfully analyzed neat API, API/lactose blends, and stressed samples.
  • Results from image analysis were comparable to those obtained using HPLC.

Conclusions:

  • Image analysis is a feasible and faster alternative for APSD determination.
  • This novel method can significantly expedite the quality assessment of inhaled therapeutics.
  • The study validates the potential of image analysis in pharmaceutical quality control.