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Updated: Apr 1, 2026
A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
Published on: December 28, 2021
Assessing spheroid viability in high throughput
Sabrina Forveille1, Flora Doffe1, Marion Leduc1
1Centre de Recherche des Cordeliers, Inserm UMRS 1138, Sorbonne Université, Université Paris Cité, Équipe labellisée par la Ligue contre le Cancer, Institut Universitaire de France, Paris, France; INSERM US23/CNRS UAR 3655, Metabolomics and Cell Biology Platforms, Institut Gustave Roussy, Université Paris-Saclay, Villejuif, France.
Abstract:
Phenotypic two-dimensional (2D) high-throughput screening (HTS) is a well-established approach extensively employed in oncological drug discovery by both Academia and the pharmaceutical industry. This methodology has played a pivotal role in the development of a wide range of systemic and targeted therapeutic anticancer agents for clinical use. Recent advances in automation, imaging technologies, and labware design have paved the way for image-based HTS in three-dimensional (3D) cell culture systems. These 3D systems enable the analysis of more physiologically relevant models that closely replicate the characteristics of tumors and their microenvironment. In this study, we present an image-based phenotypic 3D HTS assay utilizing imaging-compatible labware specifically designed to support spheroid formation.
Insights
This study introduces an advanced image-based phenotypic 3D high-throughput screening (HTS) assay. This method uses specialized labware for spheroid formation, enhancing cancer drug discovery with more relevant tumor models.
Area of Science:
- Oncology
- Drug Discovery
- Biotechnology
Background:
- Phenotypic two-dimensional (2D) high-throughput screening (HTS) is crucial for anticancer drug discovery.
- Advances in automation and imaging enable image-based HTS in 3D cell cultures.
- Three-dimensional (3D) models better mimic tumor physiology and microenvironment.
Purpose of the Study:
- To present an image-based phenotypic 3D HTS assay.
- To utilize novel imaging-compatible labware for spheroid formation.
- To advance oncological drug discovery with more relevant models.
Main Methods:
- Development of an image-based phenotypic 3D HTS assay.
- Utilization of specialized imaging-compatible labware.
- Spheroid formation for 3D cell culture models.
Main Results:
- Successful implementation of an image-based phenotypic 3D HTS assay.
- Demonstrated utility of imaging-compatible labware for spheroid culture.
- Established a more physiologically relevant platform for drug screening.
Conclusions:
- The developed 3D HTS assay offers a more accurate platform for oncological drug discovery.
- Image-based 3D screening facilitates the development of novel anticancer agents.
- This approach advances the translation of preclinical findings to clinical applications.
