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Updated: Apr 1, 2026

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

Published on: December 28, 2021

Assessing spheroid viability in high throughput.

Sabrina Forveille¹, Flora Doffe¹, Marion Leduc¹

¹Centre de Recherche des Cordeliers, Inserm UMRS 1138, Sorbonne Université, Université Paris Cité, Équipe labellisée par la Ligue contre le Cancer, Institut Universitaire de France, Paris, France; INSERM US23/CNRS UAR 3655, Metabolomics and Cell Biology Platforms, Institut Gustave Roussy, Université Paris-Saclay, Villejuif, France.

Methods in Cell Biology

|March 30, 2026

Summary

This summary is machine-generated.

This study introduces an advanced image-based phenotypic 3D high-throughput screening (HTS) assay. This method uses specialized labware for spheroid formation, enhancing cancer drug discovery with more relevant tumor models.

Keywords:

3D cell culture Compound screening Immunotherapy

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Last Updated: Apr 1, 2026

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Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Published on: August 18, 2023

Area of Science:

Oncology
Drug Discovery
Biotechnology

Background:

Phenotypic two-dimensional (2D) high-throughput screening (HTS) is crucial for anticancer drug discovery.
Advances in automation and imaging enable image-based HTS in 3D cell cultures.
Three-dimensional (3D) models better mimic tumor physiology and microenvironment.

Purpose of the Study:

To present an image-based phenotypic 3D HTS assay.
To utilize novel imaging-compatible labware for spheroid formation.
To advance oncological drug discovery with more relevant models.

Main Methods:

Development of an image-based phenotypic 3D HTS assay.
Utilization of specialized imaging-compatible labware.
Spheroid formation for 3D cell culture models.

Main Results:

Successful implementation of an image-based phenotypic 3D HTS assay.
Demonstrated utility of imaging-compatible labware for spheroid culture.
Established a more physiologically relevant platform for drug screening.

Conclusions:

The developed 3D HTS assay offers a more accurate platform for oncological drug discovery.
Image-based 3D screening facilitates the development of novel anticancer agents.
This approach advances the translation of preclinical findings to clinical applications.