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Related Concept Videos

Overview of Cell Death01:30

Overview of Cell Death

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Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
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Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
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A 3D model for assessing regulated cell death modalities.

Robin Demuynck1, Dmitri V Krysko1

  • 1Cell Death Investigation and Therapy (CDIT) Laboratory, Anatomy and Embryology Unit, Department of Human Structure and Repair, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent, Belgium.

Methods in Cell Biology
|March 30, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a high-throughput assay for screening regulated cell death (RCD) modalities, like immunogenic cell death (ICD), in intact 3D cancer models. This method avoids sample dissociation, enabling accurate drug screening and identification of novel RCD inducers.

Keywords:
CancerCell death assayFerroptosisImmunogenic cell deathImmunotherapyMultimode plate readerRegulated cell deathSpheroids

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Area of Science:

  • Biomedical Sciences
  • Cancer Research
  • Drug Discovery

Background:

  • Three-dimensional (3D) models better mimic in vivo conditions for drug screening.
  • Regulated cell death (RCD) modalities, particularly immunogenic cell death (ICD), are crucial for anti-tumor immunity.
  • Identifying specific RCD types is vital due to cancer's resistance to certain cell death pathways.

Purpose of the Study:

  • To develop a high-throughput assay for quantifying RCD modalities in intact 3D models.
  • To overcome the limitations of sample dissociation in current 3D model assays.
  • To facilitate the screening of novel RCD and ICD inducers for cancer therapy.

Main Methods:

  • Developed a protocol for analyzing RCD induction in 3D models (e.g., spheroids) without dissociation.
  • Ensured compatibility with other screening techniques like confocal microscopy.
  • Enabled high-throughput screening of cell death inducers in intact 3D models.

Main Results:

  • Successfully identified and quantified RCD modalities in intact 3D models.
  • The protocol eliminates the need for spheroid dissociation, preventing data misinterpretation.
  • Demonstrated compatibility with standard imaging techniques for RCD analysis.

Conclusions:

  • The new protocol allows for high-throughput RCD and ICD screening in 3D models.
  • This method is a critical advancement for identifying novel cancer therapeutics.
  • Accurate assessment of cell death in 3D models is now feasible for drug discovery.