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Updated: Apr 1, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
Published on: August 7, 2016
Diverse applications of volume CLEM analysis in cell biology: brief overview, unique techniques and recent notable
1Laboratory for Cryo-Electron Microscopy, Department of Molecular Cell Biology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Abstract:
The technology of volume electron microscopy (vEM), which enables three-dimensional (3D) observation of organelles, cells and even tissues at the nanoscale, is advancing and being applied to various fields of life science. As the demand for vEM grows, there is an increasing need for Volume Correlative Light and Electron Microscopy (Volume CLEM) to capture targets without missing the region of interest (ROI) or to observe the localization of molecules (often proteins) at the exact location of the fluorescent signals. This mini review provides an overview of the current state of Volume CLEM and introduces several unique approaches. My aim is to share the diverse fundamental technologies and broad application scope of this technique. vEM provides nanoscale imaging for life science applications. CLEM, is very powerful for linking molecular localization to ultrastructure. This mini review outlines advances, distinctive approaches and its broad technological and application scope.
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