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Calcium Signaling Modulator 4-MPTC Reduces Proliferative and Migration Activities of MDA-MB-468 Breast Cancer Cells

A Yu Skopin1, K O Gusev1, D A Grekhnev1

  • 1Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.

Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections
|March 30, 2026
PubMed

Insights

The compound 4-MPTC modulates calcium signaling in triple-negative breast cancer cells. It reduces cancer cell proliferation and migration, offering potential for combination therapies.

Area of Science:

  • Oncology
  • Cell Biology
  • Pharmacology

Background:

  • Malignant transformation involves cell signaling pathway remodeling, including calcium signaling.
  • Modulating calcium signals can sensitize cancer cells to chemotherapy, aiding combination therapy development.
  • Targeting calcium signaling pathways is a promising strategy for novel cancer treatments.

Purpose of the Study:

  • To investigate the effect of 4-MPTC on the invasive potential of triple-negative breast cancer (TNBC) cells.
  • To evaluate 4-MPTC as a modulator of the Stim2-dependent calcium entry pathway.
  • To assess the impact of 4-MPTC on TNBC cell proliferation and migration.

Main Methods:

  • Small molecule screening identified 4-MPTC as a Stim2-dependent calcium entry pathway modulator.
  • Experiments involved treating MDA-MB-468 TNBC cells with 4-MPTC.
  • Calcium signaling, proliferation, and migration assays were performed.

Main Results:

  • 4-MPTC induced significant calcium release from the endoplasmic reticulum (ER).
  • 4-MPTC effectively blocked store-operated calcium entry.
  • 4-MPTC treatment reduced proliferation and migration activity in MDA-MB-468 cells.

Conclusions:

  • 4-MPTC demonstrates potential as an anticancer agent by affecting calcium signaling in TNBC.
  • The compound's ability to inhibit proliferation and migration suggests its utility in combination therapies.
  • Further research into 4-MPTC could lead to new therapeutic strategies for triple-negative breast cancer.
Keywords:
breast cancercalciummigrationproliferationstore-operated entry

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