Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

3.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
3.0K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.6K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.6K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CellMate─A Deep Learning-Assisted Single-Cell Data Processing Platform.

Analytical chemistry·2026
Same author

15-PGDH inhibition preserves blood-brain barrier integrity and cognition.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Standard Addition as a Method for Quantitative Mass Spectrometry Imaging.

Analytical chemistry·2025
Same author

Spatial mapping of the AA-PGE<sub>2</sub>-EP axis in multiple sclerosis lesions.

Acta neuropathologica·2025
Same author

Endothelial-Ercc1 DNA repair deficiency provokes blood-brain barrier dysfunction.

Cell death & disease·2025
Same author

Tension at the gate: sensing mechanical forces at the blood-brain barrier in health and disease.

Journal of neuroinflammation·2024

Related Experiment Video

Updated: Apr 1, 2026

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

7.5K

Multiplexed Tandem Mass Spectrometry Imaging Enables Large-Scale Isomer Mapping and Annotation in Tissues.

Varun V Sharma1, Gabor Toth1,2, Robert Martinis1

  • 1Department of Chemistry for Life Science, Uppsala University, Uppsala, Sweden.

Angewandte Chemie (International Ed. in English)
|March 31, 2026
PubMed
Summary

We developed a new tool for molecular annotation in spatial biology using mass spectrometry imaging. This method accurately identifies molecules, including isomers, in biological tissues, advancing spatial metabolomics and chemical pathology.

Keywords:
annotationisomerslipidsmass spectrometry imagingmultiple sclerosis

More Related Videos

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

19.5K
Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
07:58

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

Published on: April 3, 2019

7.1K

Related Experiment Videos

Last Updated: Apr 1, 2026

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

7.5K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

19.5K
Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
07:58

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

Published on: April 3, 2019

7.1K

Area of Science:

  • Spatial biology
  • Mass spectrometry imaging
  • Molecular annotation

Background:

  • Accurate molecular annotation is crucial for understanding biochemical processes in spatial biology.
  • Existing methods for mass spectrometry imaging (MSI) have limitations in precise molecular identification, especially for isomers.

Purpose of the Study:

  • To present a scalable and broadly applicable molecular annotation tool for tandem mass spectrometry imaging (MS²I).
  • To enable structure-specific imaging and confident molecular annotation of MS²I data with isomeric specificity.

Main Methods:

  • Developed Parallel Image Acquisition (PIA) for simultaneous untargeted MSI and targeted MS²I.
  • Created an open-access computational framework for Spatial Similarity Networking (SSN) using graph-based spatial correlation.
  • Integrated PIA and SSN into a single workflow for enhanced molecular annotation.

Main Results:

  • Successfully visualized and annotated 134 phospholipid isomers and isobars in mouse brain tissue.
  • Mapped cholesterol metabolism in human multiple sclerosis brain tissue, identifying lesion-associated cholesterol oxidation pathways.
  • Proposed annotation confidence levels for structural annotation in MSI.

Conclusions:

  • The integrated PIA and SSN workflow provides large-scale, structure-specific MSI.
  • This approach expands the scope of spatial metabolomics, lipidomics, and chemical pathology.
  • The tool enhances molecular annotation capabilities beyond current limitations.