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Improved Methods for Recording Accurate Collision Cross Sections Using Cyclic Ion Mobility-Mass Spectrometry.

Devin M Makey1, David Langridge2, Keith Richardson2

  • 1Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.

Journal of the American Society for Mass Spectrometry
|April 1, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Accurate calibration of cyclic ion mobility (cIM) was achieved using blend and radial expressions. This method improves collision cross section (CCS) measurements in multipass and IMS^n modes, enhancing protein analysis.

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Area of Science:

  • Analytical Chemistry
  • Biophysical Chemistry
  • Separation Science

Background:

  • Traveling wave ion mobility (TWIM) is a gas-phase separation technique for analyzing protein structures and complex mixtures.
  • Collision cross section (CCS) values provide analyte size information but require calibration.
  • Existing TWIM calibration methods have limitations, especially for advanced cyclic ion mobility (cIM) applications.

Purpose of the Study:

  • To develop and validate a comprehensive method for accurate cIM calibrations using blend and radial expressions.
  • To optimize cIM calibration for multipass and IMS^n modes of operation.
  • To reduce CCS measurement error in cIM analyses.

Main Methods:

  • Applied blend and radial expressions for cIM calibration.
  • Corrected ion arrival times for time spent outside the separator.
  • Minimized velocity relaxation effects during separation.
  • Developed an optimized workflow for multipass and IMS^n cIM modes.

    • Main Results:

    • Achieved accurate cIM calibrations using blend and radial expressions.
    • Reduced CCS measurement error through arrival time corrections and minimized velocity relaxation.
    • Demonstrated the applicability of the method across various cIM operational modes.

    Conclusions:

    • The developed method provides accurate and reliable CCS measurements for cIM.
    • This approach enhances the capabilities of cIM for structural biology and complex mixture analysis.
    • Optimized calibration workflows are crucial for advancing cIM applications in IMS^n and multipass separations.