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Design and performance of an illumination-based hyperspectral laparoscopic system
Mark Witteveen1,2, Henricus J C M Sterenborg1, Theo Ruers1,2
1Surgical Department, Netherlands Cancer Institute-Antoni van Leeuwenhoek, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands.
The Review of Scientific Instruments
|April 1, 2026
Summary
This study introduces the HyperScope, a hyperspectral imaging system for laparoscopic surgery, enabling detailed tissue analysis without contrast agents. Its performance evaluation shows high spectral accuracy and acceptable spatial resolution for enhanced surgical decision-making.
Area of Science:
- Medical Imaging
- Optical Engineering
- Surgical Technology
Background:
- Hyperspectral imaging (HSI) offers advanced intraoperative tissue characterization in minimally invasive surgery (MIS).
- HSI enhances surgical decision-making by analyzing spectral signatures without exogenous contrast agents.
- Existing systems require optimization for clinical usability and spectral fidelity in surgical environments.
Purpose of the Study:
- To design and evaluate the HyperScope, an illumination-based HSI system for laparoscopic surgery.
- To assess the system's spectral and spatial resolution capabilities.
- To validate the system's potential for clinical MIS workflows.
Main Methods:
- Integration of a supercontinuum laser, tunable filter, and SWIR camera for broad spectral imaging (450-1500 nm).
- Performance evaluation through spectral response characterization, MTF measurements, and distance compensation analysis.
- Optimization of hardware and software for clinical usability and spectral fidelity.
Main Results:
- Achieved spectral deviation of 0.16 nm (450-975 nm) and -1.23 nm (1000-1675 nm).
- Demonstrated mean spatial resolutions of 0.02 mm (visible) and 0.14 mm (SWIR).
- Validated effective distance-induced spectral variability correction using SNV processing.
Conclusions:
- The HyperScope system exhibits consistent spectral accuracy and acceptable spatial resolution for MIS applications.
- The system is optimized for clinical usability and spectral fidelity.
- HyperScope shows potential for tumor detection, perfusion assessment, and real-time optical biopsies in clinical settings.
