Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Canine Fecal Microbiome Dataset: Ultra-deep Multi-platform Sequencing Across Extraction and Library Protocols.

Scientific data·2026
Same author

Veterinary Herpesviruses: Experimental Tools for Transcriptomics and Neuroscience.

Veterinary sciences·2026
Same author

Effect of long-lasting antimicrobial surface sprays in real-life environment.

BMC infectious diseases·2026
Same author

Longitudinal long-read microbiome profiling in a canine model reveals how age, diet, and birth mode shape gut community dynamics.

mSystems·2026
Same author

Genome sequence of a human monkeypox virus isolate from Central Europe during the 2022 outbreak.

Microbiology resource announcements·2025
Same author

Mapping the temporal transcriptomic signature of a viral pathogen through CAGE and nanopore sequencing.

PloS one·2025

Related Experiment Video

Updated: Apr 3, 2026

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3
07:35

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

Published on: April 13, 2009

8.6K

Multi-platform profiling reveals host- and cell -type-specific pseudorabies virus gene expression.

Balázs Kakuk1, Zsolt Csabai1, Zoltán Deim2

  • 1Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary.

Scientific Reports
|April 1, 2026
PubMed
Summary

Pseudorabies virus (PRV) transcription follows a conserved cascade, but host cells quantitatively tune its expression. This study reveals cell-type specific differences in PRV gene regulation and identifies novel viral transcripts.

More Related Videos

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

11.9K
Quantitation of Rabies Virus in Various Bovine Brain Structures
13:42

Quantitation of Rabies Virus in Various Bovine Brain Structures

Published on: May 22, 2021

4.1K

Related Experiment Videos

Last Updated: Apr 3, 2026

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3
07:35

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

Published on: April 13, 2009

8.6K
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

11.9K
Quantitation of Rabies Virus in Various Bovine Brain Structures
13:42

Quantitation of Rabies Virus in Various Bovine Brain Structures

Published on: May 22, 2021

4.1K

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Pseudorabies virus (PRV), an alphaherpesvirus, exhibits a defined transcriptional cascade (immediate-early → early → late).
  • Understanding how this cascade is modulated by different host cell types is crucial for comprehending viral infection dynamics.

Purpose of the Study:

  • To comprehensively profile PRV transcription across diverse cell types and time points.
  • To identify novel viral transcripts and analyze isoform usage during infection.
  • To elucidate host-dependent variations in the PRV transcriptional program.

Main Methods:

  • PRV transcription profiling in four cell lines (PK-15, NRK, C6, PC-12) at six time points (1-12 hpi).
  • Integration of long-read direct cDNA and direct RNA sequencing with 5'-capped CAGE-seq.
  • Differential transcript usage analysis.

Main Results:

  • Significant host-dependent transcriptional differences were observed early in infection, particularly for regulators ie180, ep0, and us1.
  • Species bias in ie180 expression and cell-type specific quantitative variations in ep0 and us1 were noted.
  • 94 previously unannotated transcripts were identified, including 5' UTR isoforms, polygenic RNAs, and noncoding RNAs.
  • Extensive isoform remodeling occurred, with a shift towards shorter isoforms during late infection.

Conclusions:

  • The canonical PRV transcriptional cascade is conserved across different cell types and hosts.
  • Host species and cell type quantitatively tune the viral transcriptional program.
  • This study provides the first multi-host, isoform-resolved temporal atlas of PRV transcription.