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A novel mouse model simulating C5 palsy after cervical surgery.
Chenpei Xu1,2,3,4,5, Xianghe Wang6,7, Mingjie Zhou1,2,3,4,5
1Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Animal Models and Experimental Medicine
|April 2, 2026
Summary
Researchers developed a new mouse model for C5 palsy (C5P) after cervical surgery. This reproducible model aids in studying permanent C5P and testing new interventions for better patient outcomes.
Area of Science:
- Neurosurgery
- Animal Modeling
- Nerve Injury Research
Background:
- C5 palsy (C5P) incidence post-cervical surgery is high (>5%) with unclear pathogenesis.
- Many patients lack satisfactory functional recovery, impacting quality of life.
- A suitable animal model for permanent C5P research is currently unavailable.
Purpose of the Study:
- To establish a novel, reproducible mouse model for permanent C5 palsy (C5P).
- To facilitate research into the pathogenesis and treatment of C5P.
- To enable exploration of new therapeutic interventions for C5P.
Main Methods:
- A posterior intradural approach was used to selectively transect the C5 ventral root in mice.
- Anatomical validation ensured precise C5 ventral root exposure while preserving the dorsal root.
- Multimodal validation included behavioral, electrophysiological, and histological assessments.
Main Results:
- The posterior intradural approach proved safe and effective, with successful survival of all mice.
- Selective C5 ventral root transection was achieved, preserving the dorsal root.
- Behavioral, electrophysiological, and histological data confirmed motor deficits and nerve/muscle pathology.
Conclusions:
- A simple, reproducible, and effective mouse model for permanent C5 palsy (C5P) has been developed.
- This model is suitable for exploring potential new therapies for C5P.
- The model supports research into nerve transfer surgery and advanced rehabilitation techniques.
