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MdSCL13 coordinates with MdTCP2 to positively regulate cold tolerance by mediating ROS scavenging and JA

Xinyue Yang1, Pengda Cheng1, Jieqiang He1

  • 1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, China.

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Summary

Apple plants’ cold tolerance is enhanced by the Scarecrow-like 13 (SCL13) protein. MdSCL13 interacts with MdTCP2 to boost reactive oxygen species detoxification and jasmonic acid accumulation under cold stress.

Keywords:
JAMdSCL13‐MdTCP2ROSapplecold

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Stress Physiology

Background:

  • Scarecrow-like (SCL) proteins are crucial in plant development.
  • The specific role of SCL13 in apple (Malus domestica) cold stress response is not well understood.

Purpose of the Study:

  • To investigate the function of MdSCL13 in apple cold tolerance.
  • To elucidate the molecular mechanism underlying MdSCL13-mediated cold response.

Main Methods:

  • RNA sequencing (RNA-seq) and RT-qPCR to analyze gene expression.
  • Co-immunoprecipitation and yeast two-hybrid assays to study protein interactions.
  • Promoter analysis to identify transcription factor binding sites.

Main Results:

  • MdSCL13 expression is induced by cold stress and positively regulates apple cold tolerance.
  • MdSCL13 influences genes involved in reactive oxygen species (ROS) detoxification and jasmonic acid (JA) biosynthesis.
  • MdSCL13 forms a complex with MdTCP2, enhancing the expression of downstream genes like MdAOC4 and MdGSTU8 through specific promoter binding by MdTCP2.

Conclusions:

  • MdSCL13 plays a vital role in enhancing apple cold tolerance.
  • The MdSCL13-MdTCP2 complex integrates hormonal signaling (JA) and redox homeostasis (ROS scavenging) for improved cold resilience.
  • This study reveals a novel GRAS protein-mediated mechanism for cold adaptation in apple.