Related Experiment Video
Updated: Mar 30, 2026

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
Published on: August 1, 2020
CALCIUM-DEPENDENT PROTEIN KINASE9 negatively regulates cold tolerance by phosphorylating the NADPH oxidase VpRBOHD in
Yuzheng Zhang1, Haoke Wang1, Xuanyang Zan1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, China.
Abstract:
Calcium-dependent protein kinases (CPKs) are key regulators of plant responses to abiotic stress; however, their roles in grapevine under cold stress remain poorly understood. In this study, we demonstrate that cold-repressed VpCPK9 and its counterpart NADPH oxidase VpRBOHD negatively regulate cold tolerance in grapevine. VpCPK9 interacts with VpRBOHD and phosphorylates VpRBOHD at Ser33, Ser132, and Ser138. This phosphorylation enhances VpRBOHD stability, thereby promoting reactive oxygen species (ROS) production under cold conditions, leading to reduced cold tolerance of grapevine. Our study reveals a negative role of the VpCPK9-VpRBOHD module in grapevine cold response and provides candidate genes for improving cold tolerance through genome editing.
More Related Videos
Related Concept Videos
Responses to Heat and Cold Stress
Adaptations that Reduce Water Loss
Introduction to Plant Diversity
Gene Regulation During Sporulation
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

